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padeasy 1 backbone plasmid  (Addgene inc)


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    Structured Review

    Addgene inc padeasy 1 backbone plasmid
    Padeasy 1 Backbone Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/padeasy+1+backbone+plasmid/pAdEasy-1+(Plasmid+%2316400)/pmc12948554-89-40-46
    Average 93 stars, based on 35 article reviews
    padeasy 1 backbone plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: DUSP26: Unveiling a critical molecular mediator and therapeutic target in developmental dysplasia of the hip‑associated secondary osteoarthritis.
    Article Snippet: .. Once constructed, the shuttle vector containing shRNAs or the rat DUSP26 CDS fragment was linearized with FastDigest PacI (cat. no. FD2204; Thermo Fisher Scientific, Inc.) and co‐transformed into Escherichia coli BJ5183 cells (Beijing Huayueyang Biological Technology Co., Ltd.) with pAdEasy‐1 backbone plasmid (cat. no. 16400; Addgene, Inc.) following the manufacturer's recommended ratio (1:1, by mass) for homologous recombination. .. The resulting recombinant adenoviral plasmids (25 μg) were then transfected into 293A packaging cells (iCell Bioscience, Inc.) using a high‐efficiency transfection reagent (cat. no. L3000015; Invitrogen; Thermo Fisher Scientific, Inc.) for 15 min at room temperature.

    Article Title: DUSP26: Unveiling a critical molecular mediator and therapeutic target in developmental dysplasia of the hip-associated secondary osteoarthritis
    Article Snippet: .. Once constructed, the shuttle vector containing shRNAs or the rat DUSP26 CDS fragment was linearized with FastDigest Pac I (cat. no. FD2204; Thermo Fisher Scientific, Inc.) and co-transformed into Escherichia coli BJ5183 cells (Beijing Huayueyang Biological Technology Co., Ltd.) with pAdEasy-1 backbone plasmid (cat. no. 16400; Addgene, Inc.) following the manufacturer's recommended ratio (1:1, by mass) for homologous recombination. .. The resulting recombinant adenoviral plasmids (25 μ g) were then transfected into 293A packaging cells (iCell Bioscience, Inc.) using a high-efficiency transfection reagent (cat. no. L3000015; Invitrogen; Thermo Fisher Scientific, Inc.) for 15 min at room temperature.

    Plasmid Preparation:

    Article Title: DUSP26: Unveiling a critical molecular mediator and therapeutic target in developmental dysplasia of the hip‑associated secondary osteoarthritis.
    Article Snippet: .. Once constructed, the shuttle vector containing shRNAs or the rat DUSP26 CDS fragment was linearized with FastDigest PacI (cat. no. FD2204; Thermo Fisher Scientific, Inc.) and co‐transformed into Escherichia coli BJ5183 cells (Beijing Huayueyang Biological Technology Co., Ltd.) with pAdEasy‐1 backbone plasmid (cat. no. 16400; Addgene, Inc.) following the manufacturer's recommended ratio (1:1, by mass) for homologous recombination. .. The resulting recombinant adenoviral plasmids (25 μg) were then transfected into 293A packaging cells (iCell Bioscience, Inc.) using a high‐efficiency transfection reagent (cat. no. L3000015; Invitrogen; Thermo Fisher Scientific, Inc.) for 15 min at room temperature.

    Article Title: DUSP26: Unveiling a critical molecular mediator and therapeutic target in developmental dysplasia of the hip-associated secondary osteoarthritis
    Article Snippet: .. Once constructed, the shuttle vector containing shRNAs or the rat DUSP26 CDS fragment was linearized with FastDigest Pac I (cat. no. FD2204; Thermo Fisher Scientific, Inc.) and co-transformed into Escherichia coli BJ5183 cells (Beijing Huayueyang Biological Technology Co., Ltd.) with pAdEasy-1 backbone plasmid (cat. no. 16400; Addgene, Inc.) following the manufacturer's recommended ratio (1:1, by mass) for homologous recombination. .. The resulting recombinant adenoviral plasmids (25 μ g) were then transfected into 293A packaging cells (iCell Bioscience, Inc.) using a high-efficiency transfection reagent (cat. no. L3000015; Invitrogen; Thermo Fisher Scientific, Inc.) for 15 min at room temperature.

    Homologous Recombination:

    Article Title: DUSP26: Unveiling a critical molecular mediator and therapeutic target in developmental dysplasia of the hip‑associated secondary osteoarthritis.
    Article Snippet: .. Once constructed, the shuttle vector containing shRNAs or the rat DUSP26 CDS fragment was linearized with FastDigest PacI (cat. no. FD2204; Thermo Fisher Scientific, Inc.) and co‐transformed into Escherichia coli BJ5183 cells (Beijing Huayueyang Biological Technology Co., Ltd.) with pAdEasy‐1 backbone plasmid (cat. no. 16400; Addgene, Inc.) following the manufacturer's recommended ratio (1:1, by mass) for homologous recombination. .. The resulting recombinant adenoviral plasmids (25 μg) were then transfected into 293A packaging cells (iCell Bioscience, Inc.) using a high‐efficiency transfection reagent (cat. no. L3000015; Invitrogen; Thermo Fisher Scientific, Inc.) for 15 min at room temperature.

    Article Title: DUSP26: Unveiling a critical molecular mediator and therapeutic target in developmental dysplasia of the hip-associated secondary osteoarthritis
    Article Snippet: .. Once constructed, the shuttle vector containing shRNAs or the rat DUSP26 CDS fragment was linearized with FastDigest Pac I (cat. no. FD2204; Thermo Fisher Scientific, Inc.) and co-transformed into Escherichia coli BJ5183 cells (Beijing Huayueyang Biological Technology Co., Ltd.) with pAdEasy-1 backbone plasmid (cat. no. 16400; Addgene, Inc.) following the manufacturer's recommended ratio (1:1, by mass) for homologous recombination. .. The resulting recombinant adenoviral plasmids (25 μ g) were then transfected into 293A packaging cells (iCell Bioscience, Inc.) using a high-efficiency transfection reagent (cat. no. L3000015; Invitrogen; Thermo Fisher Scientific, Inc.) for 15 min at room temperature.



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    Image Search Results


    Journal: Cell

    Article Title: A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2

    doi: 10.1016/j.cell.2020.08.026

    Figure Lengend Snippet:

    Article Snippet: Codon-optimized hACE2 sequences were cloned into the shuttle vector (pShuttle-CMV, Addgene 240007) to generate pShuttle-hACE2. pShuttle-hACE2 was linearized with Pme I and subsequently cotransformed with the HuAdv5 backbone plasmid (pAdEasy-1 vector; Addgene 240005) into E. coli strain BJ5183 to generate pAdV5-ACE2 by homologous recombination as described ( ).

    Techniques: Plasmid Preparation, Staining, Software, Recombinant